Biosphere laboratory (dasotrons) Joensuu

Overview

Enclosed Ecosystem Platform

Parent institution: Natural Resources Institute Finland (Luke)
Active since 2020
Active through all year

Fields of study

  • Environmental sciences, Pollution
  • Soil science
  • Terrestrial biology, Ecology
  • Other

Contact Information

Finér, Leena
email: leena.finer@luke.fi

The root laboratory is a unique facility in the European scale. It consists of four large chambers with 24 large root containers. Air and soil conditions can be controlled independently. The annual cycle of trees or herbaceous plants can be speed up, e.g. 2.5 cycles of boreal trees in one calendar year.

Environmental conditions and root and shoot responses can be monitored continuously and/or at intervals. This allows detailed studies on the dynamics of root and shoot responses, carbon sequestration and its allocation between organs in response to changing environmental factors.

The Location

Country: Finland

Latitude: 62.6

Longitude: 29.75

Nearest town: Joensuu

Distance from neaerst town: km

Relevant Research Questions

Plant responses to different environmental factors in changing and variable climate conditions in forestry and agriculture, with a special focus in root responses to different soil conditions.

Advantages

The root laboratory is a unique facility in the European scale. It consists of four large chambers with 24 large root containers. Air and soil conditions can be controlled independently. The annual cycle of trees or herbaceous plants can be speed up, e.g. 2.5 cycles of boreal trees in one calendar year. Environmental conditions and root and shoot responses can be monitored continuously and/or at intervals. This allows detailed studies on the dynamics of root and shoot responses, carbon sequestration and its allocation between organs in response to changing environmental factors.

Limitations

Root studies are very laborous. Therefore, there is a shortage of human and financial resources for running the large experiments and analyzing of the collected data. Air temperature below 0C is not possible in the chambers. The soil temperature in all 6 containers of one chamber is the same but it can different in different chambers. With special arrangements can be obtained two soil temperatures in one chamber, one is the same as air temperature and another is variable. Analysis of minirhizotron images strongly demands automatization e.g. using the approach of artificial intelligence.

Experimental Capabilities

Experimental Facilities

Six common type of growth chambers (PGW36, Conviron, Canada) and three growth rooms (GR77, Conviron, Canada) for running experiments in fully controlled and a greenhouse for semi-controlled conditions. There are instruments for studying growth, phenology and morphology of roots, physiology, anatomy, morphology, phenology and growth of aboveground parts. There is remote monitoring and programming of the chamber conditions. There are facilities for running controlled freezing tests for frost hardiness assessment of plants or their parts.

Several annual cycles of plants, including consecutive dormancy and growing seasons, can be run in one calendar year by manipulation of the environmental regimes.

Experiments duration: Weeks, Months, Years;

Experimental Aerea

Total area: 42 m²

Total area 42m2 that is divided in four chambers with six large root containers (lysimeters) in each chamber. Manipulations of air and soil regimes can be made independently in each of 4 chambers.

Available area: 70 m²

Greenhouse facility and several common type of growth chambers available (total of 160m2) (see point 14). The available space depends on the experiments of other researchers

Experimental Unit Size

unit size: 40 m²

Light canopies are movable up and down depending on the size of the plants in the experiment.

Replicates

Replicates number: 4

Depends on the experimental set-up. For air temperature and photoperiod it is four and for soil temperature five. Soil water table can be adjusted for each of 24 containers independently.

Cross-Ecosystem Manipulation

We can simulate different kind of extreme conditions and their variation in soil and air (not below 0C), and their impacts on root and shoot responses and growth of trees and herbaceous plants.

Manipulation Techniques

Temporary storage

  • Dark room
  • Drying oven
  • Freeze drier
  • Freezer
  • Liquid N
  • Refrigerator
  • Common type of growth chambers

Available Instruments

  • Minirhizotron imaging (Bartz/Vienna Scientific Instruments) and image analysis sotware (RootView)
  • Root scanner and image analysis software (WinRhizo pro)
  • Electrical impedance spectrometers (EIS-101, Agilent 4084A)
  • High pressure flow meter for root hydraulic conductance (Dynamax)
  • Chlorophyll fluorescence meter (PAM 2500)
  • Photosynthesis measurement devices (LiCor-6400, ADC LCpro)
  • Light- and stereomicroscopes with image analysis software
  • Instruments for measurement soil gases (gas chromatograph, ethylene meter)
  • Soil oxygen meters with loggers
  • Water potential meter
Electricity

Electricity available on site.

Distance from Laboratory facilities

Climate

Years measured: 40

Temperature

Mean Temperature: 3.5 °C

February Mean Temperature: -9.7 °C

July Mean Temperature: 17.2 °C

Wind

Mean Annual Speed: 4.5 m/s

Max. Annual Speed: m/s

Dominant Direction: South-West

Precipitation

Precipitation type: Rain, Snow;

Total annual precipitation: 650 mm

Mean snow cover duration: days


State Variables

Continuously Measured

  • (aquatic) Oxygen content
  • (aquatic) Water temperature
  • Air humidity
  • Air temperature
  • Canopy temperature
  • Incident PAR
  • Soil water content
  • Soil temperature

Spot Measured

  • (aquatic) Chlorophyl
  • Species richness
  • Phenology
  • Fluorescence
  • Atmospheric pressure
  • (aquatic) Nutrient levels

Environmental Pressures

Variable Ecosystem Status
CO2 Forest Planned
Warming Forest Active
Precip Forest Active
Hydro Forest Active
Management - Nutrients (levels or nutrient type) Forest Active
Management - species and/or ecotype combinations Forest Active
Management - traditional (or native) vs. new (or non-native) species Forest Planned
Warming Agrosystem Planned
Precip Agrosystem Planned
Hydro Agrosystem Planned
Management - Nutrients (levels or nutrient type) Agrosystem Planned
Management - tilling or other physical manipulation Agrosystem Planned
Management - species and/or ecotype combinations Agrosystem Planned
Warming Grassland Planned
Precip Grassland Planned
Hydro Grassland Planned
Management - Nutrients (levels or nutrient type) Grassland Planned
Management - rotation practice Grassland Planned
Management - species and/or ecotype combinations Grassland Planned
Warming Wetland Active
Precip Wetland Active
Hydro Wetland Active
Management - Nutrients (levels or nutrient type) Wetland Active
Management - species and/or ecotype combinations Wetland Active

On site Staff and Accomodation

Peak season staff

No on-site staff.

Off season staff

No on-site staff.

Visiting personnel

Up to 80 visitors


No beds on site

No showers on site

No laundry on site